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Physical Properties of Zinc Oxide. In this work we report the unfolding behavior of the periplasmic domain of the ToxR protein (ToxRp) of Vibrio cholerae on zinc oxide (ZnO) nanoparticles with a diameter of 2.5 nm. The effect of Co doping on structural and optical properties of Nanoparticles were investigated using X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) spectroscopic characterization techniques. The crystal structure for all samples was identified as purely hexagonal zinc oxide. The oxide and zinc centres are tetrahedral. the present investigation Zinc oxide nanoparticles were synthesized by sol gel method using zinc acetate and sodium hydroxide as precursors and de ionized (DI) water as solvent. Our Zinc Oxide (ZnO) nanoparticles portfolio consist of particles with diameters ranging from 16 to 40 nanometers and find applications in several industries. However, our understanding of the effect of NPs on the structure of proteins is still in a nascent state. Zinc oxide (ZnO), the most ionic of the wurtzite semiconductors, finds major applications in catalysis and as variable resistors. For zinc oxide this is often referred to as a "Wurtzite” structure (see also section 3.1.1.5). among the materials that have potential . Zinc Oxide Structure . 3. Zinc oxide (ZnO) nanoparticles had been in current studies due to its large bandwidth and high exciton binding energy and it has prospective applications such as electronic, optical, mechanical, magnetic and chemical properties that are significantly different from those of bulk counterpart. The structure and morphology of prepared zinc oxide nanoparticles was studied using X-ray diffraction (XRD) spectroscopy and Scanning Electron Microscopy (SEM). Zinc oxide nanoparticles (ZnO NPs) are used in an increasing number of industrial products such as rubber, paint, coating, and cosmetics. In the past two decades, ZnO NPs have become one of the most popular metal oxide nanoparticles in biological applications due to their excellent biocompatibility, economic, and low toxicity. It is a metal excess defect structure exhibiting n-type conductivity with a band-gap of 3.34 eV. XRD pattern of green synthesized nano-sized mono metallic zinc oxide nanoparticles powder confirmed that the formation of nano zinc oxide crystal structure in the hexagonal wurtzite phase. Zinc oxide nanoparticles are categorized . The crystallite sizes determined via Rietveld refinement characterization were as follows: Zincblende can be stabilized by growing zinc oxide on substrates which has a cubic lattice structure. ZnO nanopowders are available as powders and dispersions. Essentially, nanoparticles are a varied form of basic elements, which are derived as a result of a change to the atomic and molecular properties of the required elements. 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